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In this activity, learners separate the components of Gatorade using a home-made affinity column. In doing so, learners model the basic principle of affinity chromatography, a technique used to purify chemicals as well as bio-pharmaceuticals and petroleum products. This resource contains information about affinity chromatography and polarity.
- Under 5 minutes
- 45 to 60 minutes
- $10 - $20 per student
- Ages 11 - 18
- Activity, Experiment/Lab Activity, Lesson/Lesson Plan, Model
- English
Quick Guide
Materials List (per student)
- activated charcoal – pre-rinse with water to reduce the amount of dust
- 5ml syringe barrel for your column
- 2 paper cups
- 2 popsicle sticks
- masking tape
- paper towel or coffee filter
- hole puncher
- 3ml graduated plastic pipets
- containers to collect fractions – clear or white watercolor palettes work well
- 20% isopropanol
- 40% isopropanol
- water
- Gatorade or other colored drink (purple-colored drinks work well)
- glucose test strips (optional)
Subjects
-
Engineering and Technology
-
Engineering
- Bioengineering/Biomedical Engineering
- Chemical Engineering
-
Engineering
-
Physical Sciences
-
Chemistry
- Chemical Bonding
- Solutions
-
Vibration and Waves
- Light and Optics
-
Light and Optics
- Sunlight and Color
-
Structure and Properties of Matter
- Atomic Structure
- Elements and Periodic Table
-
Chemistry
-
The Nature of Science
-
The Scientific Process
- Conducting Investigations
- Gathering Data
- Formulating Explanations
- Communicating Results
-
The Scientific Process
Informal Categories
- Food and Cooking
- Model Building
Audience
To use this activity, learners need to:
- see
- see color
- touch
Learning styles supported:
- Involves hands-on or lab activities
Other
This resource is part of:
Access Rights:
- Free access
By:
- Yu, Julie
Rights:
- All rights reserved, Exploratorium, 2007
Funding Sources:
- National Science Foundation, 0610238
- National Science Foundation, 0925383